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KMID : 0368420190620050359
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Journal of Plant Biology
2019 Volume.62 No. 5 p.359 ~ p.373
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The Levels of Sulfur-containing Metabolites in Brassica napus are Not Influenced by the Circadian Clock but Diurnally
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Hornbacher Johann
Rumlow Annekathrin
Pallmann Philip
Turcios Ariel E.
Riemenschneider Anja
Papenbrock Jutta
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Abstract
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Adapting biological processes to an endogenous rhythm enables plants to cope with the daily changes in light and temperature in a more predictable way enhancing growth and fitness. A number of biological processes such as metabolic pathways as well as the immunity in plants are under diurnal or circadian control. In this study a possible circadian regulation of key enzymes in the sulfur assimilation and the corresponding metabolites was investigated in the agriculturally important crop plant oilseed rape (Brassica napus). Leaves of a commercially available cultivar were harvested in the course of a day under diurnal and under free-running conditions with constant light. Analyses in this study were focused on sulfur-containing metabolites and expression analysis of enzymes involved in sulfur assimilation. Expression analysis showed that the transcript levels of the sulfate transporters Sultr3;1 and Sultr4;2 as well as APR2 and APR3 oscillated diurnally. Results revealed a periodic rhythm of sulfur-containing metabolites such as glutathione, sulfate and certain glucosinolates in the course of a day which were only partly maintained under constant light. Therefore, we conclude that a diurnal rhythm and not the circadian clock regulates sulfur metabolism in plants.
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KEYWORD
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Brassica napus, Circadian clock, Diurnal rhythm, Expression analysis, Sulfur-containing metabolites
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